[sudo-discuss] [CCL] CCL: Craig's DIY Molecular Design & Cloning video

[Craig Rouskey]

Wow! Thank you for doing this Louis!

Craig


On Mon, Jan 27, 2014 at 4:25 PM, Louis Huang <louis.huang at aegia.nu> wrote:

> Hey all,
>
> Here is the video on DIY Molecular Design & Cloning by Craig that was held
> by CounterCultureLabs at sudoroom on 1/14/14. Video can be found here:
> http://www.youtube.com/watch?v=kKiqh-FhyNo
>
>
> Lecture details a basic overview of designing and cloning microorganisms
> using open access databases.
>         Subjects discussed include:
>
>                 - Primer design -- tricks, tools and resources
>                 - PCR-based cloning -- methods and means for the DIYer
>                 - Purification, Isolation and Verification
>                 - Databases and software used for design and cloning
>
>
> Join us for part II of this lecture series on cloning. Craig Rouskey will
> talk in depth on PCR:
>
>         a. PCR Principle
>         b. Components/role of each component
>         c. setting up PCR/Making adjustments to reagents
>         d. making PCR buffers (Maniatis)
>         e. Reconstituting Primers
>         f. Working the thermocycler
>
> Sign up for the event here: http://www.meetup.com/Counter-
> Culture-Labs/events/161499622/
> Held on 2/11/14 6:30PM @ sudoroom
>
> For more CounterCultureLabs events visit the meet up schedule @
> http://www.meetup.com/Counter-Culture-Labs/
>
> Happy sciencing,
> Louis
>
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