[sudo-discuss] CCL: Craig's DIY Molecular Design & Cloning video

[Louis Huang]

Hey all,

Here is the video on DIY Molecular Design & Cloning by Craig that was 
held by CounterCultureLabs at sudoroom on 1/14/14. Video can be found 
here:
http://www.youtube.com/watch?v=kKiqh-FhyNo


Lecture details a basic overview of designing and cloning 
microorganisms using open access databases.
	Subjects discussed include:

		- Primer design -- tricks, tools and resources
		- PCR-based cloning -- methods and means for the DIYer
		- Purification, Isolation and Verification
		- Databases and software used for design and cloning


Join us for part II of this lecture series on cloning. Craig Rouskey 
will talk in depth on PCR:

	a. PCR Principle
	b. Components/role of each component
	c. setting up PCR/Making adjustments to reagents
	d. making PCR buffers (Maniatis)
	e. Reconstituting Primers
	f. Working the thermocycler

Sign up for the event here: 
http://www.meetup.com/Counter-Culture-Labs/events/161499622/
Held on 2/11/14 6:30PM @ sudoroom

For more CounterCultureLabs events visit the meet up schedule @ 
http://www.meetup.com/Counter-Culture-Labs/

Happy sciencing,
Louis